Hagey Lab Secures Early Access to G4X System
June 23, 2025
Hagey Lab has secured early access to the G4X system through a significant investment from Singular Genomics; this makes Stanford Surgery one of the first sites in the country to implement this cutting-edge technology. The G4X platform offers spatial transcriptomic capabilities comparable to 10X Xenium, but with higher throughput and scalability.
Drs. Rosyli Reveron-Thornton and James Agolia, both general surgery residents currently doing their professional development time, recently completed Hagey’s first independent G4X run. The dataset they produced is the first of its kind in cholangiocarcinoma, covering 32 tissue samples on each flow cell. They are currently the only individuals at the university with the expertise to execute the full workflow, from tissue preparation to spatial data analysis.
“This project required nearly a full week of intensive work and represents an impressive technical accomplishment,” said Dr. Dan Delitto, an assistant professor in the Division of General Surgery. “I think their success reflects the strength of our surgical residency training model and the depth of scientific engagement made possible through department and division funds as well as Hagey's infrastructure. Their performance demonstrates that surgical residents can match PhD-trained scientists in mastering complex genomic technologies and contributing to high-level scientific discoveries.”
The Delitto Lab was awarded a Department Research Instrumentation Grant in 2024, which enabled the purchase of a Chromium X & G4 Sequencer.
“As some perspective, when I completed my PhD in 2016, microarrays were considered a major advance, producing a few thousand data points per sample,” said Delitto. “Today, we are working with single cell multiomic and spatial platforms that generate billions of data points per sample.”
In the coming months, Reveron-Thorton and Agolia will train fellow General Surgery Residents Drs. Chuner Guo and Maria Korah, who are already developing new pipelines focused on chromatin accessibility and in silico drug discovery.
“The manuscripts are forthcoming, but the genomic and informatic skills these residents are building will prepare them to navigate and contribute to a rapidly evolving field in the future,” said Delitto.
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