Synergistic Use of Cell-Assisted Lipotransfer and Allograft Adipose Matrix to Improve Fat Graft Volume Retention

Introduction: Cell-Assisted Lipotransfer (CAL) utilizing Stromal Vascular Fraction (SVF) has been shown to significantly enhance graft volume retention in autograft lipotransfer. Additionally, Allograft Adipose Matrices (AAM), such as “Renuva,” have been shown to prolong graft longevity and stimulate adipose tissue regeneration. This study investigates the synergistic effects of combining CAL with AAM to optimize fat graft outcomes.

Methods: Twenty adult female NU/J nude immunodeficient mice were divided into four groups and injected with one of the following treatments: SVF-enhanced adipose tissue, non-enhanced adipose tissue, AAM, or a combination of both. The groups were: (1) SVF-treated adipose tissue, (2) non-treated adipose tissue, (3) non-treated adipose tissue with AAM, and (4) SVF-treated adipose tissue with AAM. High-resolution 3T Bruker MRI scans were performed every two weeks for twelve weeks to monitor graft volume and tissue changes.

Results: Image analysis revealed significant differences between groups. Group 2 showed the poorest volume retention, while Group 3 demonstrated progressively better outcomes. Groups 4 and 1 exhibited the highest volume retention, with group 4 showing the most substantial retention.

Conclusion: These results highlight the probable or possible benefits of combining CAL and AAM in enhancing autograft volume retention. This approach offers insights into improving the quality and longevity of fat grafts.